Key Parameters of Optical Microscopes：Resolution

The resolution of an optical microscope refers to the minimum distance between two object points that the microscope can clearly distinguish. Also known as the resolving power, it reflects the microscope's ability to distinguish fine details. The maximum resolution of a typical optical microscope is approximately 0.2 micrometers.

The formula for resolution is:

σ = λ / NA

Where:

· σ is the minimum resolvable distance.

· λ is the wavelength of light.

· NA is the numerical aperture of the objective lens.

How to Improve Resolution:

1.Use a light source with a shorter wavelength to reduce the value of λ.

2.Increase the refractive index (n) of the medium or the aperture angle (α) to raise the NA value.

3.Adjust the imaging conditions of the microscope to enhance contrast, thereby improving resolution.